ABSTRACT
Objective To study the association between CYP2C9 gene polymorphism and warfarin maintenance dosage in anticoagulation therapy.Methods 200 Han patients admitted to our hospital for heart valve replacement were included in this study.CYP2C9 * 2,CYP2C9 * 3,CYP2C9 *c65 in CYP2C9 gene were sequenced using the CAPS technique and conventional DNA sequencing method.Dosages of warfarin used in patients carrying different genes were analyzed.Results No mutation of CYP2C9 * 2 but only one kind of allele C was detected in 200 patients.The genotype of CYP2C9 * 2 was C/C wild type.Allelic gene was detected at CYP2C9 * 3 A and C,with A/A wild type detected in 171 patients,A/C heterozygote mutation type detected in 18 patients,and C/C heterozygote mutation type detected in 11 patients respectively.The frequency of allelic genes A and B was 94.3 ％ and 5.7 ％ respectively.A significant difference was found between CYP2C9 * 3 mutation and warfarin dosage (P＜0.05).The dosage of warfarin reduced 18.46％ and 76.0％ respectively in patients carrying A/C heterozygote mutation type and in those carrying C/C heterozygote mutation type.Two kinds of allelic gene were detected at CYP2C9 * c65 G and C,with G/G wild type detected in 182 patients and G/C heterozygote mutation type detected in 18 patients respectively.No significant association was found in warfarin maintenance dosage for patients carrying G/G wild type and G/C heterozygote mutation type.Conclusion CYP2C9 gene polymorphism is associated with warfarin maintenance dosage in anticoagulation therapy.
ABSTRACT
OBJECTIVE@#To evaluate the clinical effect of posterior decompression and open-door laminoplasty combined with lateral mass plate fixation in the treatment of ossification of the posterior longitudinal ligament of the cervical spine (OPLL).@*METHODS@#A total of 41 OPLL patients in our hospital, with differing extents of spinal compression, were analyzed retrospectively. All cases underwent a posterior open-door laminoplasty operation, and lateral mass plate fixation. The Japanese Orthopedic Association ( JOA) score was adopted to evaluate nerve function.@*RESULTS@#All cases were followed up for 12 to 16 (14±2.0) months. The postoperative JOA score was increased 4.8 points compared with the preoperative.@*CONCLUSION@#Posterior open-door laminoplasty combined with lateral mass plate fixation for ossification of the posterior longitudinal ligament of the cervical spine possesses the advantages of extensive applicability, simplicity and safety, and strong stability. It is an efficient and reliable method for ossification of the posterior longitudinal ligament.